Skip to content

Short-Read Sequencing

Definition
AI-generated

Short-read sequencing generates relatively short DNA reads, typically tens to a few hundred bases long, at high throughput and low per-base cost. It is the dominant format for most large-scale WGS and WES studies.

Topics
Sequencing and omics tech
Related
Sequencing by synthesis (SBS) Long-Read Sequencing Paired-End Sequencing

Why it matters in GWAS

Short-read platforms are efficient for common-variant discovery and reference panel generation, but can be weaker for repetitive regions, structural variants, and long-range haplotype resolution.

Example usage

"Most participants were analyzed with short-read sequencing, while a smaller subset received long-read follow-up."

Sequencing by synthesis (SBS) Long-Read Sequencing Paired-End Sequencing Read Whole-Exome Sequencing (WES) Whole-Genome Sequencing (WGS)
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Metzker ML. (2010). Sequencing technologies: the next generation. Nat Rev Genet.
← Sex-linked Short Tandem Repeat (STR) →

Last updated (UTC · Git history)