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Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Definition
AI-generated

CUT&RUN maps chromatin-associated proteins or modifications with an antibody-targeted nuclease that cleaves and releases DNA locally; released fragments are sequenced, yielding high signal-to-noise profiles with low cell inputs compared with some ChIP-seq workflows.

Topics
Sequencing and omics tech
Synonyms
CUT&RUN
Related
ATAC-seq ChIP-seq Chromatin

Why it matters in GWAS

Epigenomic marks and factor binding annotate regulatory hypotheses for noncoding GWAS hits; method choice affects resolution and cell-type feasibility in functional follow-up.

Example usage

"CUT&RUN for H3K27ac prioritized enhancers overlapping the fine-mapped interval."

ATAC-seq ChIP-seq Chromatin Enhancer Hi C
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Skene PJ, Henikoff S. (2017). An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife.
← Cumulative minor allele count (cMAC) Cleavage Under Targets and Tagmentation (CUT&Tag) →

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