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Unique Molecular Identifier (UMI)

Definition
AI-generated

A unique molecular identifier (UMI) is a random molecular barcode added to cDNA before PCR in many single-cell RNA-seq protocols.

Topics
Sequencing and omics tech
Synonyms
UMI
Related
Ambient RNA Coverage RNA Sequencing (RNA-seq)

Why it matters in GWAS

Accurate per-cell count matrices underpin clustering, pseudobulk aggregation, and eQTL models; UMI-aware counting reduces technical noise that can obscure genetic effects.

Example usage

"We collapsed reads by UMI and cell barcode to build a sparse count matrix for downstream analysis."

Ambient RNA Coverage RNA Sequencing (RNA-seq) Read Depth Single-Cell Sequencing
Related-term network

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Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Cuomo ASE, Nathan A, Raychaudhuri S, et al. (2023). Single-cell genomics meets human genetics. Nat Rev Genet. https://doi.org/10.1038/s41576-023-00599-5
  • Islam S, et al. (2014). Quantitative single-cell RNA-seq with unique molecular identifiers. Nat Methods.
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