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Perturb-seq

Definition
AI-generated

Perturb-seq denotes pooled CRISPR (or related) screens in which each cell carries a genetic perturbation and is profiled by single-cell RNA sequencing, yielding genome-scale maps of how knocking out or activating genes shifts transcriptional phenotypes.

Topics
Single-cell analysis
Synonyms
Perturb-seq screening
Related
Gene Regulatory Network Inference In Silico Perturbation Prediction scPerturb

Why it matters in GWAS

When a locus points to several plausible effector genes, Perturb-seq-style data can show which perturbations recapitulate disease-relevant programs in a relevant model cell type—orthogonal evidence to eQTL overlap alone.

Example usage

"The prioritized gene appeared as a strong hit in a published Perturb-seq map of immune stimulation responses."

Gene Regulatory Network Inference In Silico Perturbation Prediction scPerturb Single-Cell Perturbation Genomics Single-Cell Sequencing
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Dixit A, et al. (2016). Perturb-Seq: dissecting molecular circuits with scalable single-cell RNA profiling of pooled genetic screens. Cell.
  • Replogle JM, et al. (2022). Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq. Cell.
  • Dimitrov D, Schrod S, Rohbeck M, et al. (2026). Interpretation, extrapolation and perturbation of single cells. Nat Rev Genet. https://doi.org/10.1038/s41576-025-00920-4
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