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PCR amplification

Definition
AI-generated

PCR amplification is the exponential enzymatic copying of a DNA or cDNA template between two primers over thermal cycles, enlarging a specific locus or library segment before genotyping, cloning, or sequencing.

Topics
Molecular biology Sequencing and omics tech
Related
Polymerase chain reaction (PCR) PCR duplicate Primer

Why it matters in GWAS

Genotyping panels, targeted resequencing, and many library preparations rely on PCR; amplification skew and duplicates affect allele balance, depth interpretation, and artifact filtering. Some protocols (e.g. certain CAGE variants) deliberately omit PCR to reduce bias when mapping ends or low-complexity regions.

Example usage

"After PCR amplification, unique molecular identifiers were used to collapse duplicate reads before allele counting."

Polymerase chain reaction (PCR) PCR duplicate Primer Unique molecular identifier
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References

  • Griffiths AJF, et al. (2015). An Introduction to Genetic Analysis. W. H. Freeman.
← Pathway Analysis PCR duplicate →

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