Skip to content

Sequencing artifact

Definition
AI-generated

Sequencing artifacts are non-biological patterns in reads or base calls—such as oxidation effects (OxoG), adapter chimeras, or instrument-specific errors—that can masquerade as variants.

Topics
Sequencing Data Analysis
Related
Base quality GC bias Variant Quality Score Recalibration (VQSR)

Why it matters in GWAS

Artifact filters and recalibration reduce false positives in WGS call sets that would otherwise enter rare-variant tests.

Example usage

"OxoG sequencing artifact metrics were reviewed for FFPE-like damage before retaining calls."

Base quality GC bias Variant Quality Score Recalibration (VQSR)
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Costello M, et al. (2013). Characterization and remediation of systematic errors in sequencing data. Genome Biol.
← Sequencing Sex-linked →

Last updated (UTC · Git history)