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Doublet in Single-Cell Sequencing

Definition
AI-generated

A doublet (multiplet) is two or more cells captured and barcoded as one observation in a single-cell experiment.

Topics
Single-cell analysis
Synonyms
Multiplet
Related
Ambient RNA Genetic Demultiplexing Quality Control (QC)

Why it matters in GWAS

Doublets add noise to single-cell and pseudobulk analyses and can confuse demultiplexing and cell-state models; aggressive filtering is standard before sc-eQTL or integration with GWAS.

Example usage

"Putative Doublet in Single-Cell Sequencing barcodes were removed from single-cell data before clustering and eQTL mapping."

Ambient RNA Genetic Demultiplexing Quality Control (QC) Single-Cell Sequencing
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References

  • Cuomo ASE, Nathan A, Raychaudhuri S, et al. (2023). Single-cell genomics meets human genetics. Nat Rev Genet. https://doi.org/10.1038/s41576-023-00599-5
  • McCarthy DJ, et al. (2017). Scater: pre-processing, quality control, normalization and visualization of single-cell RNA-seq data in R. Bioinformatics.
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