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Sanger Sequencing

Definition
AI-generated

Sanger sequencing uses chain-terminating dideoxynucleotides and capillary electrophoresis to read a single DNA sequence fragment at a time, historically the gold standard for accuracy and still common for validation.

Topics
Sequencing and omics tech
Related
Genotyping Array Single Nucleotide Polymorphism (SNP) Variant Calling

Why it matters in GWAS

Sanger is often used to confirm candidate variants from GWAS or sequencing pipelines, design primers, and validate rare or clinically important alleles.

Example usage

"Lead SNPs were genotyped by Sanger sequencing in an independent replication cohort."

Genotyping Array Single Nucleotide Polymorphism (SNP) Variant Calling Whole-Genome Sequencing (WGS)
Related-term network

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References

  • Sanger F, Nicklen S, Coulson AR. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA.
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