Skip to content

Single-molecule real-time sequencing (SMRT)

Definition
AI-generated

PacBio single-molecule real-time (SMRT) sequencing reads DNA in long continuous fragments; HiFi reads combine long length with high accuracy and are widely used for assembly, phasing, and structural variant detection.

Topics
Sequencing and omics tech
Synonyms
SMRT sequencing HiFi sequencing
Related
Long-Read Sequencing Structural Variant Variant Calling

Why it matters in GWAS

PacBio HiFi data improves resolution of structural variation, haplotypes, and difficult regions when validating or extending GWAS hits beyond short-read arrays and imputation.

Example usage

"A replication analysis checks whether assumptions tied to Single-molecule real-time sequencing (SMRT) hold across cohorts."

Long-Read Sequencing Structural Variant Variant Calling Whole-Genome Sequencing (WGS)
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Wenger AM, et al. (2019). Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nat Biotechnol.
← P value Pacific Islander →

Last updated (UTC · Git history)