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N50

Definition
AI-generated

N50 is a contiguity statistic: the length L such that contigs (or scaffolds) of length ≥ L cover at least half of the total assembly length; larger N50 usually indicates a more continuous genome assembly under fixed total span.

Topics
Bioinformatics Sequencing Data Analysis
Related
Assembly gap De novo assembly Genome assembly

Why it matters in GWAS

Assembly quality influences structural variant detection, novel sequence inclusion, and reference genome patches that eventually propagate to SNP arrays and imputation panels used in association studies.

Example usage

"The phased assembly reached 40 Mb N50 with long reads and Hi-C scaffolding."

Assembly gap De novo assembly Genome assembly Haplotype-resolved assembly
Related-term network

Click any node to open that term page.

Node order. The current term is first. Next come every page linked from Related terms, in the same top-to-bottom order as that list. Then new terms appear in two-hop layers: for each of those direct neighbors (in that same order), their related terms are appended if not already listed. A third pass does the same for two-hop nodes. The walk stops when a fixed maximum number of nodes is reached.

References

  • Earl D, et al. (2011). Assemblathon 1: a competitive assessment of de novo short read assembly methods. Genome Res.
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